Skip to Content
Find More Like This
Return to Search

Method and apparatus for biological material separation

United States Patent

6,890,740
May 10, 2005
View the Complete Patent at the US Patent & Trademark Office
Los Alamos National Laboratory - Visit the Technology Transfer Division Website
There has been invented an apparatus comprising a separation barrier for excluding denser cell materials from less dense cell materials after centrifuging of the cells so that selected materials can be withdrawn from the less dense cell materials without inclusion of the denser cell materials or clogging of sampling equipment with denser cell materials. Cells from which selected material is to be withdrawn are centrifuged, either as cells or cells in media. Once the denser cell materials are isolated in a layer by centrifugal force, an invention screen or seive is submerged in the less dense cell material to a level above the layer of denser cell materials to isolate the denser cell materials from the less dense cell materials, preventing mixing of the denser cell materials back into the less dense cell materials when the cells or the cells in media are no longer being centrifuged and to prevent clogging of sampling equipment with denser cell materials. In a particularly useful application of the invention method and apparatus, plasmid DNA can be withdrawn from less dense cell materials without contamination or interference with denser cell materials.
Robinson; Donna L. (Los Alamos, NM)
The Regents of the University of California (Los Alamos, NM)
09/ 782,324
20020110923
February 12, 2001
This invention was made with government support under Contract No. W-7405-ENG-36 awarded by the U.S. Department of Energy. The government has certain rights in the invention.