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System and method for introduction and stabilization of genes in Thermus sp.

United States Patent

March 1, 2005
View the Complete Patent at the US Patent & Trademark Office
A method for introducing and stabilizing heterologous and recombinant genes in a thermophilic host in which a characteristic gene defining a detectable host characteristic is inactivated or deleted from the thermophilic host, resulting in a modified thermophilic host expressing an absence of the detectable host characteristic. A DNA fragment of interest is inserted into the modified thermophilic host together with an intact characteristic gene, whereby the detectable host characteristic is restored to the thermophilic host, thereby enabling detection and confirmation of successful transformation using plasmid vectors and integration of the DNA fragment into the chromosome of the thermophilic host.
Kayser; Kevin J. (Woodstock, IL), Park; Ho-Shin (Chicago, IL), Kilbane, II; John J. (Woodstock, IL)
Gas Technology Institute (Des Plaines, IL)
10/ 085,388
February 28, 2002
This invention was made with Government support under Prime Contract No. DE-FG02-97ER62464 awarded by the Department of Energy. The Government has certain rights in this invention.