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System and method of infrared matrix-assisted laser desorption/ionization mass spectrometry in polyacrylamide gels

United States Patent

November 30, 2004
View the Complete Patent at the US Patent & Trademark Office
A system and method for desorption and ionization of analytes in an ablation medium. In one embodiment, the method includes the steps of preparing a sample having analytes in a medium including at least one component, freezing the sample at a sufficiently low temperature so that at least part of the sample has a phase transition, and irradiating the frozen sample with short-pulse radiation to cause medium ablation and desorption and ionization of the analytes. The method further includes the steps of selecting a resonant vibrational mode of at least one component of the medium and selecting an energy source tuned to emit radiation substantially at the wavelength of the selected resonant vibrational mode. The medium is an electrophoresis medium having polyacrylamide. In one embodiment, the energy source is a laser, where the laser can be a free electron laser tunable to generate short-pulse radiation. Alternatively, the laser can be a solid state laser tunable to generate short-pulse radiation. The laser can emit light at various ranges of wavelength.
Haglund, Jr.; Richard F. (Brentwood, TN), Ermer; David R. (Mississippi State, MS), Baltz-Knorr; Michelle Lee (Nashville, TN)
Vanderbilt University (Nashville, TN)
09/ 931,490
August 16, 2001
This invention was made with Government support under a contract awarded by the U.S. Department of Navy and a contract awarded by the U.S. Department of Energy, respectively, and the Government has certain rights in this invention.