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Chemical inducible promoter used to obtain transgenic plants with a silent marker

United States Patent

August 31, 2004
View the Complete Patent at the US Patent & Trademark Office
A chemically inducible promoter is described that may be used to transform plants, including tobacco and lettuce, with genes which are easily regulatable by adding the plants or plant cells to a medium containing an inducer of the promoter or by removing the plants or plant cells from such medium. The promoter described is one that is inducible by a glucocorticoid which is not endogenous to plants. Such promoters may be used with a variety of genes such as ipt or knotted1 to induce shoot formation in the presence of a glucocorticoid. The promoter may also be used with antibiotic or herbicide resistance genes which are then regulatable by the presence or absence of inducer rather than being constitutive. Other examples of genes which may be placed under the control of the inducible promoter are also presented.
Aoyama; Takashi (Shiga, JP), Zuo; Jianru (New York, NY), Chua; Nam-Hai (Scarsdale, NY)
The Rockefeller University (New York, NY)
09/ 438,392
November 12, 1999
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH This application was made in part with Government support under DOE Grant #FG02-94ER20142, funded by the Department of Energy.