Fluorescent biosensor molecules, fluorescent biosensors and systems, as well as methods of making and using these biosensor molecules and systems are described. Embodiments of these biosensor molecules exhibit fluorescence emission at wavelengths greater than about 650 nm. Typical biosensor molecules include a fluorophore that includes an iminium ion, a linker moiety that includes a group that is an anilinic type of relationship to the fluorophore and a boronate substrate recognition/binding moiety, which binds glucose. The fluorescence molecules modulated by the presence or absence of polyhydroxylated analytes such as glucose. This property of these molecules of the invention, as well as their ability to emit fluorescent light at greater than about 650 nm, renders these biosensor molecules particularly well-suited for detecting and measuring in-vivo glucose concentrations.
This invention was made with United States Government support under CRADA number 70NANB8H4065 awarded by the National Institute of Standards and Technology (NIST). The United States Government has certain rights in the invention.
Portions of this work have been performed under the auspices of the U.S. Department of Energy by the University of California, Lawrence Livemore National Laboratory under Contract No. W-7405-ENG-48. The government may have certain rights to this invention.