Skip to Content
Find More Like This
Return to Search

Engineering of living cells for the expression of holo-phycobiliprotein-based constructs

United States Patent

May 25, 2004
View the Complete Patent at the US Patent & Trademark Office
Recombinant cells which express a fluorescent holo-phycobiliprotein fusion protein and methods of use are described. The cells comprises a bilin, a recombinant bilin reductase, an apo-phycobiliprotein fusion protein precursor of the fusion protein comprising a corresponding apo-phycobiliprotein domain, and a recombinant phycobiliprotein domain-bilin lyase, which components react to form the holo-phycobiliprotein fusion protein. Also described are holo-phycobiliprotein based transcription reporter cells and assays, which cells conditionally express a heterologous-to-the-cell, fluorescent, first holo-phycobiliprotein domain.
Glazer; Alexander N. (Berkeley, CA), Tooley; Aaron J. (Berkeley, CA), Cai; Yuping (Carmel, IN)
The Regents of the University of California (Oakland, CA)
09/ 919,486
July 31, 2001
This invention was made with Government support under contract DE-FG-9161125 awarded by the U.S. Department of Energy. The Government has certain rights in this invention.