The present invention provides methods for the production of proteins, particularly toxic proteins, in host cells. The invention provides methods which use a fusion protein comprising a chaperonin binding domain in host cells induced or regulated to have increased levels of chaperonin which binds the chaperonin binding domain.
FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT
This invention was made with Government support under Grant No. 70NANB5H1138 awarded by Advanced Technology Program/National Institute of Standards and Technology. The Government has certain rights in this invention.
This Invention was made with Government support under ANL Work-for-Others Proposal No. P-95090; Prime Contract W-31-198-ENG-38 awarded by the Department of Energy. The Government has certain rights In this Invention.