Skip to Content
Find More Like This
Return to Search

Nucleotide sequences encoding pinoresinol/lariciresinol reductase proteins and their methods of use

United States Patent

October 21, 2003
View the Complete Patent at the US Patent & Trademark Office
Dirigent proteins and pinoresinol/lariciresinol reductases have been isolated, together with cDNAs encoding dirigent proteins and pinoresinol/lariciresinol reductases. Accordingly, isolated DNA sequences are provided from source species Forsythia intermedia, Thuja plicata, Tsuga heterophylla, Eucommia ulmoides, Linum usitatissimum, and Schisandra chinensis, which code for the expression of dirigent proteins and pinoresinol/lariciresinol reductases. In other aspects, replicable recombinant cloning vehicles are provided which code for dirigent proteins or pinoresinol/lariciresinol reductases or for a base sequence sufficiently complementary to at least a portion of dirigent protein or pinoresinol/lariciresinol reductase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding dirigent protein or pinoresinol/lariciresinol reductase. Thus, systems and methods are provided for the recombinant expression of dirigent proteins and/or pinoresinol/lariciresinol reductases.
Lewis; Norman G. (Pullman, WA), Davin; Laurence B. (Pullman, WA), Dinkova-Kostova; Albena T. (Baltimore, MD), Fujita; Masayuki (Kita-gun, JP), Gang; David R. (Ann Arbor, MI), Sarkanen; Simo (Minneapolis, MN), Ford; Joshua D. (Pullman, WA)
Washington State University Research Foundation (Pullman, WA)
09/ 704,640
November 2, 2000
GOVERNMENT RIGHTS This invention was funded in part by grant number DE-FG03-97ER20259 from the United States Department of Energy, by grant number MCB09631980 from the National Science Foundation, by grant number NAG100164 from the National Aeronautics and Space Administration, and by grant number 96-35103-3358 from the United States Department of Agriculture. The government has certain rights in this invention.