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Method for altering antibody light chain interactions

United States Patent

6,485,943
November 26, 2002
View the Complete Patent at the US Patent & Trademark Office
Argonne National Laboratory - Visit the Technology Development and Commercialization Website
A method for recombinant antibody subunit dimerization including modifying at least one codon of a nucleic acid sequence to replace an amino acid occurring naturally in the antibody with a charged amino acid at a position in the interface segment of the light polypeptide variable region, the charged amino acid having a first polarity; and modifying at least one codon of the nucleic acid sequence to replace an amino acid occurring naturally in the antibody with a charged amino acid at a position in an interface segment of the heavy polypeptide variable region corresponding to a position in the light polypeptide variable region, the charged amino acid having a second polarity opposite the first polarity. Nucleic acid sequences which code for novel light chain proteins, the latter of which are used in conjunction with the inventive method, are also provided.
Stevens; Fred J. (Naperville, IL), Stevens; Priscilla Wilkins (Evanston, IL), Raffen; Rosemarie (Elmhurst, IL), Schiffer; Marianne (Downers Grove, IL)
The University of Chicago (Chicago, IL)
09/ 274,163
20020137897
March 22, 1999
The United States Government has rights in this invention pursuant to Contract No. W-31-109-ENG-38 between the U.S. Departrnent of Energy (DOE) and the University of,Chicago representing Argonne National Laboratory.