Skip to Content
Find More Like This
Return to Search

Triplex in-situ hybridization

United States Patent

October 8, 2002
View the Complete Patent at the US Patent & Trademark Office
Disclosed are methods for detecting in situ the presence of a target sequence in a substantially double-stranded nucleic acid segment, which comprises: a) contacting in situ under conditions suitable for hybridization a substantially double-stranded nucleic acid segment with a detectable third strand, said third strand being capable of hybridizing to at least a portion of the target sequence to form a triple-stranded structure, if said target sequence is present; and b) detecting whether hybridization between the third strand and the target sequence has occured.
Fresco; Jacques R. (Princeton, NJ), Johnson; Marion D. (East Windsor, NJ)
Princeton University (Princeton, NJ)
09/ 531,000
September 8, 2000
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT The present work was supported by grants from the National Institutes of Health (GM42936) and the Department of Energy (DE-FG02-96ER62202).