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Glycoconjugates and methods

United States Patent

October 1, 2002
View the Complete Patent at the US Patent & Trademark Office
Lawrence Berkeley National Laboratory - Visit the Technology Transfer and Intellectual Property Management Department Website
Methods for making the functionalized glycoconjugates include (a) contacting a cell with a first monosaccharide, and (b) incubating the cell under conditions whereby the cell (i) internalizes the first monosaccharide, (ii) biochemically processes the first monosaccharide into a second saccharide, (iii) conjugates the saccharide to a carrier to form a glycoconjugate, and (iv) extracellularly expresses the glycoconjugate to form an extracellular glycoconjugate comprising a selectively reactive functional group. Methods for forming products at a cell further comprise contacting the functional group of the extracellularly expressed glycoconjugate with an agent which selectively reacts with the functional group to form a product. Subject compositions include cyto-compatible monosaccharides comprising a nitrogen or ether linked functional group selectively reactive at a cell surface and compositions and cells comprising such saccharides.
Bertozzi; Carolyn (Albany, CA), Yarema; Kevin J. (Albany, CA), Mahal; Lara K. (Berkeley, CA)
The Regents of the University of California (Oakland, CA)
09/ 571,527
May 16, 2000
The research carried out in the subject application was supported in part by grants from the Department of Energy (Contract No. DE-AC03-76SF00098). The government may have rights in any patent issuing on this application.