Skip to Content
Find More Like This
Return to Search

Encapsulation of biomaterials in porous glass-like matrices prepared via an aqueous colloidal sol-gel process

United States Patent

October 16, 2001
View the Complete Patent at the US Patent & Trademark Office
The present invention provides a process for the encapsulation of biologically important proteins into transparent, porous silica matrices by an alcohol-free, aqueous, colloidal sol-gel process, and to the biological materials encapsulated thereby. The process is exemplified by studies involving encapsulated cytochrome c, catalase, myoglobin, and hemoglobin, although non-proteinaceous biomaterials, such as active DNA or RNA fragments, cells or even tissues, may also be encapsulated in accordance with the present methods. Conformation, and hence activity of the biomaterial, is successfully retained after encapsulation as demonstrated by optical characterization of the molecules, even after long-term storage. The retained conformation of the biomaterial is strongly correlated to both the rate of gelation and the subsequent drying speed of the encapsulatng matrix. Moreover, in accordance with this process, gelation is accelerated by the use of a higher colloidal solid concentration and a lower synthesis pH than conventional methods, thereby enhancing structural stability and retained conformation of the biomaterials. Thus, the invention also provides a remarkable improvement in retaining the biological activity of the encapsulated biomaterial, as compared with those involved in conventional alkoxide-based processes. It further provides new methods for the quantitative and qualitative detection of test substances that are reactive to, or catalyzed by, the active, encapsulated biological materials.
Liu; Dean-Mo (Richmond, CA), Chen; I-Wei (Swarthmore, PA)
The Trustees of the University of Pennsylvania (
09/ 661,122
September 13, 2000
GOVERNMENT INTERESTS This invention was supported in part by the U.S. Department of Energy (BES) under Grant No. DE-FG02-97ER45637 A001. The Government may have certain rights in this invention.