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Nucleic acid amplification using modular branched primers

United States Patent

May 22, 2001
View the Complete Patent at the US Patent & Trademark Office
Argonne National Laboratory - Visit the Technology Development and Commercialization Website
Methods and compositions expand the options for making primers for use in amplifying nucleic acid segments. The invention eliminates the step of custom synthesis of primers for Polymerase Chain Reactions (PCR). Instead of being custom-synthesized, a primer is replaced by a combination of several oligonucleotide modules selected from a pre-synthesized library. A modular combination of just a few oligonucleotides essentially mimics the performance of a conventional, custom-made primer by matching the sequence of the priming site in the template. Each oligonucleotide module has a segment that matches one of the stretches within the priming site.
Ulanovsky; Levy (Westmont, IL)
University of Chicago (Chicago, IL)
09/ 264,466
March 8, 1999
The U.S. Government has rights to the invention pursuant to Contract W-31-109-ENG between the U.S. Department of Energy and the University of Chicago representing Argonne National Laboratory.