A system for changing the gloves of a glove box. The system requires the use of a new glove and a glove change ring to form a temporary secondary barrier to the exchange of atmospheres between the inner glove box and the room in which the glove box is operated. The system describes specific means for disengaging a used glove from the glove box port. The means for disengaging the used glove include use of a glove change hook and use of a glove with an attached tab for use in removal. A method for changing the gloves of a glove box is also described.