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Orpinomyces cellulase CelE protein and coding sequences

United States Patent

6,110,720
August 29, 2000
View the Complete Patent at the US Patent & Trademark Office
A CDNA designated celE cloned from Orpinomyces PC-2 encodes a polypeptide (CelE) of 477 amino acids. CelE is highly homologous to CelB of Orpinomyces (72.3% identity) and Neocallimastix (67.9% identity), and like them, it has a non-catalytic repeated peptide domain (NCRPD) at the C-terminal end. The catalytic domain of CelE is homologous to glycosyl hydrolases of Family 5, found in several anaerobic bacteria. The gene of celE is devoid of introns. The recombinant proteins CelE and CelB of Orpinomyces PC-2 randomly hydrolyze carboxymethylcellulose and cello-oligosaccharides in the pattern of endoglucanases.
Li; Xin-Liang (Athens, GA), Ljungdahl; Lars G. (Athens, GA), Chen; Huizhong (Athens, GA)
University of Georgia Research Foundation, Inc. (Athens, GA)
09/ 118,324
July 17, 1998
ACKNOWLEDGEMENT OF FEDERAL RESEARCH SUPPORT This invention was made, at least in part, with funding from the United States Department of Energy (Grant No. DE-FG05 93ER 20127). Accordingly, the United States Government has certain rights in this invention.