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Recombinant cells that highly express chromosomally-integrated heterologous genes

United States Patent

6,107,093
August 22, 2000
View the Complete Patent at the US Patent & Trademark Office
Recombinant host cells are obtained that comprise (A) a heterologous, polypeptide-encoding polynucleotide segment, stably integrated into a chromosome, which is under transcriptional control of an endogenous promoter and (B) a mutation that effects increased expression of the heterologous segment, resulting in enhanced production by the host cells of each polypeptide encoded by that segment, relative to production of each polypeptide by the host cells in the absence of the mutation. The increased expression thus achieved is retained in the absence of conditions that select for cells displaying such increased expression. When the integrated segment comprises, for example, ethanol-production genes from an efficient ethanol producer like Zymomonas mobilis, recombinant Escherichia coli and other enteric bacterial cells within the present invention are capable of converting a wide range of biomass-derived sugars efficiently to ethanol.
Ingram; Lonnie O. (Gainesville, FL), Ohta; Kazuyoshi (Gainesville, FL), Wood; Brent E. (Gainesville, FL)
University of Florida Research Foundation, Inc. (Gainesville, FL)
09/ 134,403
August 14, 1998
GOVERNMENT SUPPORT Work relating to this invention was supported in part by Grant FG05-86ER3574 from the Office of Basic Energy Science, U.S. Department of Energy, and in part by Grant 88-37233-3987 from the Alcohol Fuels Program, U.S. Department of Agriculture. The U.S. Government has certain rights in the invention.