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Use of continuous/contiguous stacking hybridization as a diagnostic tool

United States Patent

6,090,549
July 18, 2000
View the Complete Patent at the US Patent & Trademark Office
Argonne National Laboratory - Visit the Technology Development and Commercialization Website
A method for detecting disease-associated alleles in patient genetic material is provided whereby a first group of oligonucleotide molecules, synthesized to compliment base sequences of the disease associated alleles is immobilized on a predetermined position on a substrate, and then contacted with patient genetic material to form duplexes. The duplexes are then contacted with a second group of oligonucleotide molecules which are synthesized to extend the predetermined length of the oligonucleotide molecules of the first group, and where each of the oligonucleotide molecules of the second group are tagged and either incorporate universal bases or a mixture of guanine, cytosine, thymine, and adenine, or complementary nucleotide strands that are tagged with a different fluorochrome which radiates light at a predetermined wavelength. The treated substrate is then washed and the light patterns radiating therefrom are compared with predetermined light patterns of various diseases that were prepared on identical substrates. A method is also provided for determining the length of a repeat sequence in DNA or RNA, and also for determining the base sequence of unknown DNA or RNA.
Mirzabekov; Andrei Darievich (Moscow, RU), Kirillov; Eugene Vladislavovich (Moscow, RU), Parinov; Sergei Valeryevich (Moscow, RU), Barski; Victor Evgenievich (Moscow, RU), Dubiley; Svetlana Alekseevna (Ukraine, RU)
University of Chicago (
08/ 855,372
May 13, 1997
CONTRACTUAL ORIGIN OF THE INVENTION The United States Government has rights in this invention pursuant to Contract No. W-31-109-ENG-380 between the U.S. Department of Energy and the University of Chicago representing Argonne National Laboratory.