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Dyes designed for high sensitivity detection of double-stranded DNA

United States Patent

6,054,272
April 25, 2000
View the Complete Patent at the US Patent & Trademark Office
Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a cationic chain. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.
Glazer; Alexander N. (Orinda, CA), Benson; Scott C. (Oakland, CA)
The Regents of the University of California (Berkeley, CA)
08/ 965,917
November 7, 1997
This invention was made with Government support under Grant Contract No. BE-FG-91ER61125 awarded by the Department of Energy. The Government has certain rights to this invention.