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In vitro gastrointestinal mimetic protocol for measuring bioavailable contaminants

United States Patent

6,040,188
March 21, 2000
View the Complete Patent at the US Patent & Trademark Office
Lawrence Berkeley National Laboratory - Visit the Technology Transfer and Intellectual Property Management Department Website
The present invention relates to measurements of contaminants in the soil and other organic or environmental materials, using a biologically relevant chemical analysis that will measure the amount of contaminants in a given sample that may be expected to be absorbed by a human being ingesting the contaminated soil. According to the present invention, environmental samples to be tested are added to a pre-prepared physiological composition of bile salts and lipids. They are thoroughly mixed and then the resulting mixture is separated e.g. by centrifugation. The supernatant is then analyzed for the presence of contaminants and these concentrations are compared to the level of contaminants in the untreated samples. It is important that the bile salts and lipids be thoroughly pre-mixed to form micelles.
Holman; Hoi-Ying N. (Berkeley, CA)
The Regents of the University of California (Oakland, CA)
09/ 196,618
November 18, 1998
STATEMENT REGARDING FEDERAL SPONSORSHIP This invention was made with U.S. Government support under Contract No. DE-AC03-76SF0098 between the U.S. Department of Energy and the University of California for the operation of Lawrence Berkeley National Laboratory (LBNL). The U.S. Government may have certain rights to this invention.