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Methods for immobilizing nucleic acids on a gel substrate

United States Patent

5,981,734
November 9, 1999
View the Complete Patent at the US Patent & Trademark Office
Argonne National Laboratory - Visit the Technology Development and Commercialization Website
A method for labeling oligonucleotide molecules, and for immobilizing oligonucleotide and DNA molecules is provided comprising modifying the molecules to create a chemically active group, and contacting activated fluorescent dyes to the region. A method for preparing an immobilization substrate is also provided comprising modifying a gel to contain desired functional groups which covalently interact with certain moieties of the oligonucleotide molecules. A method for immobilizing biomolecules and other molecules within a gel by copolymerization of allyl-substituted oligonucleotides, DNA and proteins with acrylamide is also provided.
Mirzabekov; Andrei Darievich (Moscow, RU), Proudnikov; Dimitri Y. (Samara, RU), Timofeev; Edward N. (Moscow, RU), Kochetkova; Svetlana V. (Moscow, RU), Florentiev; Vladimir L. (Moscow, RU), Shick; Valentine V. (Moscow, RU)
University of Chicago (Chicago, IL)
08/ 895,656
July 17, 1997
The United States Government has rights in this invention pursuant to Contract No. W-31-109-ENG-38 between the U.S. Department of Energy and the University of Chicago representing Argonne National Laboratory.