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On-line DNA analysis system with rapid thermal cycling

United States Patent

5,935,522
August 10, 1999
View the Complete Patent at the US Patent & Trademark Office
Oak Ridge National Laboratory - Visit the Partnerships Directorate Website
An apparatus particularly suited for subjecting biological samples to any necessary sample preparation tasks, subjecting the sample to rapid thermal cycling, and then subjecting the sample to subsequent on-line analysis using one or more of a number of analytical techniques. The apparatus includes a chromatography device including an injection means, a chromatography pump, and a chromatography column. In addition, the apparatus also contains a capillary electrophoresis device consisting of a capillary electrophoresis column with an inlet and outlet end, a means of injection, and means of applying a high voltage to cause the differential migration of species of interest through the capillary column. Effluent from the liquid chromatography column passes over the inlet end of the capillary electrophoresis column through a tee structure and when the loading of the capillary electrophoresis column is desired, a voltage supply is activated at a precise voltage and polarity over a specific duration to cause sample species to be diverted from the flowing stream to the capillary electrophoresis column. A laser induced fluorescence detector preferably is used to analyze the products separated while in the electrophoresis column.
Swerdlow; Harold P. (Salt Lake City, UT), Wittwer; Carl T. (Salt Lake City, UT)
University of Utah Research Foundation (Salt Lake City, UT)
08/ 885,625
June 30, 1997
This invention was made with Government support under Contract No. DE-AC05-760R00033 awarded by the Department of Energy and Contract No. 5P30HG00199 awarded by the National Institutes of Health. The Government has certain rights in this invention.