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Time-resolved fluorescence decay measurements for flowing particles

United States Patent

5,909,278
June 1, 1999
View the Complete Patent at the US Patent & Trademark Office
Los Alamos National Laboratory - Visit the Technology Transfer Division Website
Time-resolved fluorescence decay measurements for flowing particles. An apparatus and method for the measurement and analysis of fluorescence for individual cells and particles in flow are described, wherein the rapid measurement capabilities of flow cytometry and the robust measurement and analysis procedures of time-domain fluorescence lifetime spectroscopy are combined. A pulse-modulated cw laser is employed for excitation of the particles. The characteristics and the repetition rate of the excitation pulses can be readily adjusted to accommodate for fluorescence decays having a wide range of lifetimes.
Deka; Chiranjit (Miami, FL), Steinkamp; John A. (Los Alamos, NM)
The Regents of the University of California (Los Alamos, NM)
08/ 902,395
July 29, 1997
The present invention relates generally to fluorescence decay measurements and, more particularly, to the measurement of the time-resolved fluorescence decay for flowing particles excited by suitable light sources. This invention was made with government support under Contract No. W-7405-ENG-36 awarded by the U.S. Department of Energy to The Regents of the University of California. The government has certain rights in the invention.