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Endoglucanase mutants and mutant hydrolytic depolymerizing enzymes and uses thereof

United States Patent Application

20030054535
A1
View the Complete Application at the US Patent & Trademark Office
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The invention provides a method for increasing the specific activity of a glycosyl hydrolase on a substrate, comprising replacing a hydrophobic surface binding amino acid of the hydrolase with a positively charged amino acid; and a method for increasing the specific activity of a glycosyl hydrolase on a substrate, comprising replacing an active site associated glycosyl-stabilizing amino acid of the hydrolase with an amino acid, the replacing amino acid not strongly retarding cellobiose from leaving the active site. The invention further provides mutant glycosyl hydrolases, which include Y245G, Y42R, and W82R.
Himmel, Michael E. (Littleton, CO), Adney, William S. (Golden, CO), Baker, John O. (Golden, CO), Vinzant, Todd B. (Golden, CO), Thomas, Steven R. (Denver, CO), Sakon, Joshua (Fayetteville, AR), Decker, Stephen R. (Berthoud, CO)
09/ 997,504
November 19, 2001
[0002] The United States Government has rights in this invention pursuant to Contract No. DE-AC36-99GO-10337 between the United States Department of Energy and the Midwest Research Institute.