A process for extracting an aqueous ammonium hydroxide solution from a plant biomass after an Ammonia Fiber Explosion (AFEX) process step, is described. The proteins can be separated before or after a hydrolysis of sugar precursors (carbohydrates) from the biomass to produce sugars for fermentation to produce ethanol. The proteins are useful as animal feeds because of their amino acid food values.
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT
 This research was under a grant from the United States Department of Energy (USDOE) Contract No. DE-FG36-04GO-14220. THE U.S. GOVERNMENT HAS CERTAIN RIGHTS TO THIS INVENTION.