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High-throughput methods of distinguishing at least one molecule individually in a sample comprising multiple molecules and systems for use therein

United States Patent Application

20020167665
A1
View the Complete Application at the US Patent & Trademark Office
Ames Laboratory - Visit the Iowa State University Research Foundation - Office of Intellectual Property & Technology Transfer Website
A high-throughput method of distinguishing at least one molecule individually in a sample comprising multiple molecules, which method comprises: subjecting a sample comprising multiple molecules, at least one molecule of which is detectably labeled, to electrophoresis; imaging the electrophoretic mobility of each detectably labeled molecule over time by detecting the position of the detectable label of each detectably labeled molecule over time, and, optionally, at the same time, dispersing the imaging by a transmission grating for spectroscopic analysis, and determining the electrophoretic mobility of each detectably labeled molecule and, optionally, determining the molecular spectrum of each detectably labeled molecule, thereby distinguishing at least one molecule individually in a sample comprising multiple molecules, and a system for use in such a method. A second high-throughput method of distinguishing at least one molecule individually in a sample comprising multiple molecules, which method comprises: introducing a sample comprising multiple molecules in free solution, at least one molecule of which is detectably labeled, into a sample channel; simultaneously imaging the position of each detectably labeled molecule, by detecting the position of the detectable label of each detectably labeled molecule, and dispersing the imaging by a transmission grating for spectroscopic analysis, and determining the molecular spectrum of each detectably labeled molecule, thereby distinguishing at least one molecule individually in a sample comprising multiple molecules, and a system for use in such a method.
Yeung, Edward S. (Ames, IA), Shortreed, Michael (Portage, WI), Ma, Yinfa (Rolla, MO)
10/ 031,353
April 11, 2002
[0001] This invention was made with government support under Department of Energy Contract No. W-7405-Eng-82 and National Institutes of Health Contract Nos. RO 1 CA76961-04A1 and 5R01 CA76961-02. Therefore, the government of the U.S.A. may have certain rights in this invention.