Methods and apparatus for implementing microfluidic analysis devices are provided. A monolithic elastomer membrane associated with an integrated pneumatic manifold allows the placement and actuation of a variety of fluid control structures, such as structures for pumping, isolating, mixing, routing, merging, splitting, preparing, and storing volumes of fluid. The fluid control structures can be used to implement a variety of sample introduction, preparation, processing, and storage techniques.
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH
 The techniques and mechanisms of the present invention were made with Government support under Contract DEFG91ER61125 by the US Department of Energy, by NASA Grant No. NAG5-9659, and by NIH grants HG01399 and P01 CA 77664.