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METHOD OF MAKING GEL DROP PROTEIN BIOCHIPS

United States Patent Application

20080241944
A1
View the Complete Application at the US Patent & Trademark Office
Argonne National Laboratory - Visit the Technology Development and Commercialization Website
A method of making a gel drop protein chip by transferring proteins, which were obtained from a cellular lysate, partitioned using two-dimensional, protein fractionation, and mixed with a polymeric matrix solution containing acrylamide/bis and glycerol, to an array; a method of making a gel drop protein chip by transferring proteins, which were derivatized with N-hydroxysuccinimide ester of N-methacryloyl-6-aminocaproic acid (NHS monomer), and mixed with a polymeric matrix solution containing acrylamide/bis and glycerol, to an array; a gel drop protein chip containing proteins in a polymeric matrix solution containing acrylamide/bis glycerol; a method of using the gel drop protein chip to interrogate a sample; and a protein derivatized with the NHS monomer.
Schabacker, Daniel S. (Naperville, IL)
UCHICAGO ARGONNE, LLC (Chicago IL)
11/ 693,523
March 29, 2007
STATEMENT OF GOVERNMENTAL SUPPORT [0001] This invention was partially conceived under Contract No. W-31-109-ENG-38 between the U.S. Department of Energy and the University of Chicago representing Argonne National Laboratory. This invention was supported, at least in part, with funding from the Department of Energy under Agreement/Award No. 03A18. Therefore, the Government of the United States of America has certain rights in the invention.