The present invention relates to methods, cells and compositions for performing in vivo recombination without the need for clonal selection. These methods, cells and compositions are useful for the automation of recombination. The present invention provides methods for performing sequential steps in genome engineering, as well as for achieving parallel and multiplex genome engineering.
STATEMENT OF GOVERNMENT INTERESTS
 This invention was made with government support under the U.S. Department of Energy GTL grant number DE-FG02-02ER63445. The Government has certain rights in the invention.