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Method rubisco large subunit n-methyltransferase useful for targeting molecules to the active-site vicinity of ribulose-1, 5-bisphosphatet

United States Patent Application

20070169213
A1
View the Complete Application at the US Patent & Trademark Office
The present invention generally relates to a modified Rubisco large subunit .sup..epsilon.N-Methyltransferase (Rubisco LSMT, or RLSMT). The present invention also relates to a modified RLSMT-carbonic anhydrase (RLSMT-CA). This modified RLSMT-CA improves the efficiency of the reduction of CO.sub.2 during photosynthesis, which may increase plant growth rates. The present invention also relates to nucleic acids encoding the modified RLSMT-CA or modified RLSMT. Also, the present invention relates to cells including the modified RLSMT-CA or modified RLSMT, plants containing the modified RLSMT-CA or modified RLSMT, and methods using compositions of the present invention. In addition, the present invention relates to antibodies conjugated to CA which may bind to Rubisco, and antibodies which bind a modified RLSMT-CA. The invention also relates to modified forms of the LS and SS of Rubisco where the modified forms are fusions with CA or biologically active fragments thereof. The present invention provides methods of altering Rubisco carboxylase activity and altering plant growth.
Houtz, Robert L. (Lexington, KY)
University of Kentucky Research Foundation (Intellectual Property Department, A144 Astecc Bldg Lexington KY 40506-0286)
10/ 555,795
May 7, 2004
[0002] This work was supported by the U.S. Department of Energy grant DE-FG02-02-92ER20075, and therefore the government may have certain rights to the invention.