A method is provided for acquiring X-ray diffraction images of a protein crystal, the method comprising providing an X-ray source and a micro-channel, wherein the micro-channel contains an oil, gas or immiscible liquid and at least one aqueous droplet comprising a crystal of a protein, recording an X-ray diffraction image of the crystal, repositioning the crystal with respect to the X-ray source, and repeating the last two steps immediately above at least once.
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT
 This work was supported at least in part with funds from the U.S. Government under U.S.P.H.S. Grants Y1-CO-1020 awarded by the National Cancer Institute, Y1-GM-1 104 awarded by the National Institute of General Medical Sciences, GM073197 EB001903 awarded by the National Institutes of Health, and under Contract No. W-31-109-Eng-38 from the U.S. Department of Energy. The U.S. Government has certain rights in the invention.