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MULTIPLEX CHARACTERIZATION OF MICROBIAL TRAITS USING DUAL BARCODED NUCLEIC ACID FRAGMENT EXPRESSION LIBRARY

United States Patent Application

20180030435
A1
View the Complete Application at the US Patent & Trademark Office
Lawrence Berkeley National Laboratory - Visit the Technology Transfer and Intellectual Property Management Department Website
Disclosed herein are barcoded expression libraries comprising a plurality of expression vectors, wherein each expression vector comprises a nucleic acid fragment flanked by a first barcode and a second barcode. Further disclosed herein are methods of making the barcoded expression libraries and methods of conducting functional analysis using the barcoded expression libraries.
Mutalik, Vivek K. (Albany, CA), Deutschbauer, Adam (Berkeley, CA), Arkin, Adam P. (San Francisco, CA), Novichkov, Pavel (Walnut Creek, CA)
15/ 665,226
July 31, 2017
STATEMENT REGARDING FEDERALLY SPONSORED R&D [0002] This invention was made with government support under grant Nos. DE-SC0008812 and DE-AC02-05CH11231 awarded by the U.S. Department of Energy. The government has certain rights in the invention.