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Modulating Laccase Enzyme to Regulate Cell Wall Biosynthesis and Recalcitrance in Plants

United States Patent Application

20180016592
A1
View the Complete Application at the US Patent & Trademark Office
Oak Ridge National Laboratory - Visit the Partnerships Directorate Website
This disclosure provides genetically modified plants having desirable levels of sugar release and syringyl/guaiacyl (S/G) ratio; methods of genetically modifying plants to modulate sugar release and S/G ratio; and uses of such plants. The inventors have determined that genetic modification of a laccase gene (LAC2) from Populus, encoded by locus Potri.008G064000 resulted in transgenic Populus trees with changes in syringyl/guaiacyl ratios as well as altered sugar release phenotypes. Plants with altered sugar release, and S/G ratio, based on modulation of the expression or activity of the LAC2 gene, have divergent uses including pulp and paper production, and biofuel and bioproducts production.
CHEN, Jin-Gui (Oak Ridge, TN), GUNTER, Lee E. (Oak Ridge, TN), JAWDY, Sara S. (Oak Ridge, TN), YANG, Xiaohan (Knoxville, TN), TUSKAN, Gerald A. (Oak Ridge, TN), BRYAN, Anthony Christian (Oak Ridge, TN)
15/ 647,819
July 12, 2017
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT [0002] This disclosure was made with government support under a research project supported by Prime Contract No. DE-AC05-00OR22725 awarded by the U.S. Department of Energy. The government has certain rights in this invention.