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METHODS FOR GENERATING BARCODED COMBINATORIAL LIBRARIES

United States Patent Application

20170369870
A1
View the Complete Application at the US Patent & Trademark Office
Provided herein are methods and composition for trackable genetic variant libraries. Further provided herein are methods and compositions for recursive engineering. Further provided herein are methods and compositions for multiplex engineering. Further provided herein are methods and compositions for enriching for editing and trackable engineered sequences and cells using nucleic acid-guided nucleases.
GILL, Ryan T. (Denver, CO), GARST, Andrew (Boulder, CO), LIPSCOMB, Tanya Elizabeth Warnecke (Boulder, CO), BASSALO, Marcelo Colika (Boulder, CO), ZEITOUN, Ramsey Ibrahim (San Francisco, CA)
15/ 632,222
June 23, 2017
STATEMENT AS TO FEDERALLY SPONSORED RESEARCH [0002] This disclosure was made with the support of the United States government under Contract number DE-SC0008812 by the Department of Energy.