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TYROSINE HYDROXYLASE VARIANTS AND METHODS OF USE THEREOF

United States Patent Application

20170306301
A1
View the Complete Application at the US Patent & Trademark Office
The present disclosure provides a variant tyrosine hydroxylase that provides for increased production of L-DOPA in a host cell that expresses the tyrosine hydroxylase. The present disclosure provides nucleic acids encoding the variant tyrosine hydroxylase, and host cells genetically modified with the nucleic acids. The present disclosure provides methods of making L-DOPA in a host cell. The present disclosure provides methods of making a benzylisoquinoline alkaloid (BIA), or a BIA precursor. The present disclosure provides methods of detecting L-DOPA level in a cell. The present disclosure provides methods of identifying tyrosine hydroxylase variants that provide for increased L-DOPA production; and methods of identifying gene products that provide for increased tyrosine production.
Martin, Vincent J. J. (Montreal, CA), Narcross, Lauren (Montreal, CA), Dueber, John E. (San Francisco, CA), DeLoache, William C. (Oakland, CA), Russ, Zachary N. (Berkeley, CA), Scrivens, P. James (St. Constant, CA)
The Regents of the University of California (Oakland CA)
15/ 507,711
September 24, 2015
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH [0002] This invention was made with government support under Grant No. DE-SC9998084 awarded by the Department of Energy. The government has certain rights in the invention.