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BIOPRODUCT FORMATION FROM A PLASMID ADDICTION SYSTEM IN THE ABSENCE OF CO-INDUCERS AND ANTIBIOTICS

United States Patent Application

20170306337
A1
View the Complete Application at the US Patent & Trademark Office
Described herein are plasmid addiction systems comprising a host cell comprising one or more inactivated host cell essential genes; and a plasmid comprising one or more plasmid essential genes operably linked to a constitutively active promoter. Also described herein are metabolism-based plasmid addiction systems (PAS) comprising a host cell and a plasmid operably linked to a constitutively active promoter for producing value-based products (e.g., 1-butanol) and methods of generating PASs in microorganisms and producing 1-butanol from a PAS in the absence of antibiotics and/or co-inducers.
TABITA, F. Robert (Dublin, OH), LAGUNA, Rick Avalos (Columbus, OH), YOUNG, Sarah Jeanne (Gahanna, OH)
15/ 528,301
November 19, 2015
STATEMENT REGARDING FEDERALLY FUNDED RESEARCH [0002] This invention was made with government support under grant number DE-AR000009 awarded by U.S. Department of Energy (FRT and STY). The government has certain rights in the invention.