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METHODS FOR PRENYLATION OF PEPTIDES AND THEIR USE IN OVER-PRODUCTION OF FARNESENE AND GERANYLGERANYL TERPENES

United States Patent Application

20170240926
A1
View the Complete Application at the US Patent & Trademark Office
Methods for concentrating biofuel precursors, including terpenes such as farnesyl and geranylgeranyl derivatives, are based on the prenylation of peptides in living organisms, such as plant or algae cells. Generally, an expression vector containing a gene encoding a small peptide with a preferred amino acid sequence is used to produce a transgenic organism. Expression of the gene in the cells produces a short peptide which is processed by the protein prenylation machinery of the cell. This results in a peptide-prenyl fusion in which a sesqui- or di-terpene molecule is attached to the peptide. Due to its small size and amphiphilic properties, this molecule forms micelles which allow the sesqui- or di-terpene to accumulate to high concentrations within the cell. The peptide-prenyl micelles are then extracted and purified for use preferably as a biofuel.
McQualter, Richard (Denton, TX)
15/ 436,402
February 17, 2017
[0002] The present invention used in part funds from the U.S. Department of Energy, Advanced Research Projects Agency, Grant No. DE-AR0000208. The United States Government has certain rights in the invention.