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COMPOSITIONS AND METHODS FOR SINGLE-MOLECULE CONSTRUCTION OF DNA

United States Patent Application

20170218416
A1
View the Complete Application at the US Patent & Trademark Office
Lawrence Berkeley National Laboratory - Visit the Technology Transfer and Intellectual Property Management Department Website
The present invention provides for compositions and methods for single-molecule construction of DNA. The present invention provides for a method comprising: (a) providing a reaction chamber comprising a solid support bound to a single starter double-stranded (ds) DNA molecule comprising a free end, (b) introducing one or more extension molecules and one or more enzymes capable of joining a payload region of an extension molecule to the free end of starter dsDNA molecule to the reaction chamber wherein the extension molecule comprises an cleavable linker.
Arlow, Daniel H. (Berkeley, CA), Holtz, William J. (Oakland, CA), Weaver, Lane J. (San Francisco, CA), Keasling, Jay D. (Berkeley, CA)
The Regents of the University of California (Oakland CA)
15/ 311,846
May 18, 2015
STATEMENT OF GOVERNMENTAL SUPPORT [0002] This invention was made with government support under Contract No. DE-AC02-05CH11231 awarded by the United States Department of Energy and Award No. EEC-0540879 awarded by the National Science Foundation. The government has certain rights in the invention.