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Bardcoded Protein Array for Multiplex Single-Molecule Interaction Profiling

United States Patent Application

20170107566
A1
View the Complete Application at the US Patent & Trademark Office
Methods for attaching barcodes to polypeptides are provided. Methods for detecting molecular interactions at the single molecule level are provided. Embodiments of the invention are directed to a ONA barcoded protein array technology for parallel protein interaction profiling on a single molecule basis. DNA barcodes are attached to proteins collectively via ribosome display or individually via enzymatic conjugation. Novel methods are described herein that measure protein interactions based on the statistical analysis of co-localized polonies arising from barcoding DNAs of interacting proteins.
Church, George M. (Brookline, MA), Gu, Liangcai (Boston, MA)
15/ 128,145
March 25, 2015
STATEMENT OF GOVERNMENT INTERESTS [0002] This invention was made with government support under DE-FG02-02ER63445 awarded by the U.S. Department of Energy. The government has certain rights in this invention.