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Compositions and Methods for Making Selenocysteine Containing Polypeptides

United States Patent Application

View the Complete Application at the US Patent & Trademark Office
Non-naturally occurring tRNA.sup.Sec and methods of using them for recombinant expression of proteins engineered to include one or more selenocysteine residues are disclosed. The non-naturally occurring tRNA.sup.Sec can be used for recombinant manufacture of selenocysteine containing polypeptides encoded by mRNA without the requirement of an SECIS element. In some embodiments, selenocysteine containing polypeptides are manufactured by co-expressing a non-naturally occurring tRNA.sup.Sec a recombinant expression system, such as E. coli, with SerRS, EF-Tu, SelA, or PSTK and SepSecS, and an mRNA with at least one codon that recognizes the anticodon of the non-naturally occurring tRNA.sup.Sec.
Soll, Dieter (Guilford, CT), Aldag, Caroline (New Haven, CT), Hohn, Michael (Scotch Plains, NJ), Miller, Corwin (New Haven, CT)
15/ 202,194
July 5, 2016
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT [0002] This invention was made with government support under GM022854 awarded by National Institute of Health, DE-FG02-98ER20311 awarded by the Department of Energy and 0950474 awarded by the National Science Foundation. The government has certain rights in the invention.