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METHODS OF PLANT TRANSFORMATION USING TRANSFORMABLE CELL SUSPENSION CULTURE AND USES THEREOF

United States Patent Application

20170130236
A1
View the Complete Application at the US Patent & Trademark Office
The subject invention pertains to a method of rapidly transforming a plant with a gene of interest. The method of the current invention comprises the steps of a) preparing a bacterial culture, wherein the bacterial culture comprises a vector containing the gene of interest, b) producing a plant cell suspension culture from the plant, c) contacting the plant cell suspension culture with the bacterial culture to produce a plant cell transformed with the gene of interest from the plant cell suspension culture, and e) producing a plant from the plant cell transformed with the gene of interest. The method of the current invention can be designed for a high throughput transformation and screening of a plant with a plurality of genes of interest and screening the plants to identify and obtain plants having desirable characteristics.
STEWART, Jr., C. NEAL (KNOXVILLE, TN), WILLIS, JONATHAN D. (KNOXVILLE, TN), BURRIS, JASON (KNOXVILLE, TN), MAZAREI, MITRA (KNOXVILLE, TN)
15/ 311,199
June 2, 2015
[0002] This invention was made with government support under 0670-2176 awarded by Advanced Research Projects Agency-Energy, U.S. Department of Energy. The government has certain rights in the invention.