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METHOD AND APPARATUS FOR MEASURING BIOFILM THICKNESS AND TOPOLOGY

United States Patent Application

20160272933
A1
View the Complete Application at the US Patent & Trademark Office
Pacific Northwest National Laboratory - Visit the Technology Commercialization Program Website
An apparatus and method of measuring biofilm and biological activity on a surface is disclosed. The apparatus includes a biofilm, which includes one or more microorganisms, grown on a substrate. A viewing window is placed on a surface of the biofilm and a gas bubble is introduced between the viewing window and the surface of the biofilm. The space between the substrate and the viewing window may be enclosed in a casing that has an inlet and an outlet, forming a flow cell. A microscope system, such as a white light interferometer, captures data of the biofilm in situ and non-destructively. The 3D images of biofilm surface have high resolution while maintaining a large field of view. This invention will be useful for fundamental studies of biofilms, biomedical and environmental screening, and many other applications in biology and the life sciences.
Larimer, Curtis J. (Richland, WA), Suter, Jonathan D. (Richland, WA), Addleman, Raymond S. (Benton City, WA), Bonheyo, George T. (Sequim, WA), Brann, Michelle R. (Richland, WA)
BATTELLE MEMORIAL INSTITUTE (Richland WA)
15/ 071,921
March 16, 2016
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH OR DEVELOPMENT [0002] This invention was made with Government support under Contract DE-AC0576RL01830 awarded by the U.S. Department of Energy. The Government has certain rights in the invention.