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Engineering Cyclopropane Fatty Acid Accumulation in Plants

United States Patent Application

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Heterologous expression of E. coli cyclopropane synthase in genotypic and phenotypic fad2fae1 plants facilitates accumulation of cyclopropane fatty acids. Co-expression of Sterculia foetida transferases, including lysophosphatidic acid acyltransferase, diacylglycerol acyltransferase (DGAT), and Phospholipid Diacyl Glycerol Acyltransferase (PDAT), with E. coli cyclopropane synthase further enhances cyclopropane fatty acid accumulation in fad2fae1 plant seeds.
Shanklin, John (Shoreham, NY), Yu, Xiao-Hong (Mount Sinai, NY), Prakash, Richa Rawat (Medford, NY)
Brookhaven Science Associates, LLC (Upton NY)
14/ 914,532
August 26, 2014
GOVERNMENT SUPPORT [0002] This invention was made with U.S. Government support under contract number DE-AC02-98CH10886, awarded by the U.S. Department of Energy, and Grant DBI 0701919 awarded by the National Science Foundation. The U.S. Government has certain rights in the invention.