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United States Patent Application

View the Complete Application at the US Patent & Trademark Office
Pacific Northwest National Laboratory - Visit the Technology Commercialization Program Website
This disclosure provides Agrobacterium-mediated transformation methods for the oil-producing (oleaginous) yeast Lipomyces sp., as well as yeast produced by the method. Such methods utilize Agrobacterium sp. cells that have a T-DNA binary plasmid, wherein the T-DNA binary plasmid comprises a first nucleic acid molecule encoding a first protein and a second nucleic acid molecule encoding a selective marker that permits growth of transformed Lipomyces sp. cells in selective culture media comprising an antibiotic.
Dai, Ziyu (Richland, WA), Magnuson, Jon K. (Richland, WA), Deng, Shuang (Richland, WA), Bruno, Kenneth S. (Richland, WA), Culley, David E. (Richland, WA)
Battelle Memorial Institute (Richland WA)
14/ 540,818
November 13, 2014
ACKNOWLEDGMENT OF GOVERNMENT SUPPORT [0001] This invention was made with government support under Contract DE-AC0576RLO1830 awarded by the U.S. Department of Energy. The government has certain rights in the invention.