Skip to Content
Find More Like This
Return to Search

MICROORGANISMS AND METHODS FOR PRODUCING PYRUVATE, ETHANOL, AND OTHER COMPOUNDS

United States Patent Application

20160068871
A1
View the Complete Application at the US Patent & Trademark Office
Microorganisms comprising modifications for producing pyruvate, ethanol, and other compounds. The microorganisms comprise modifications that reduce or ablate activity of one or more of pyruvate dehydrogenase, 2-oxoglutarate dehydrogenase, phosphate acetyltransferase, acetate kinase, pyruvate oxidase, lactate dehydrogenase, cytochrome terminal oxidase, succinate dehydrogenase, 6-phosphogluconate dehydrogenase, glutamate dehydrogenase, pyruvate formate lyase, pyruvate formate lyase activating enzyme, and isocitrate lyase. The microorganisms optionally comprise modifications that enhance expression or activity of pyruvate decarboxylase and alcohol dehydrogenase. The microorganisms are optionally evolved in defined media to enhance specific production of one or more compounds. Methods of producing compounds with the microorganisms are provided.
Reed, Jennifer L. (Madison, WI), Zhang, Xiaolin (Newark, DE)
Wisconsin Alumni Research Foundation (Madison WI)
14/ 848,646
September 9, 2015
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH [0001] This invention was made with government support under DE-FC02-07ER64494, DE-SC0008103 awarded by the US Department of Energy. The government has certain rights in the invention.