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RAPID DISCOVERY AND SCREENING OF ENZYME ACTIVITY USING MASS SPECTROMETRY

United States Patent Application

20150330992
A1
View the Complete Application at the US Patent & Trademark Office
Lawrence Berkeley National Laboratory - Visit the Technology Transfer and Intellectual Property Management Department Website
Described herein are methods, systems, and compositions for detecting enzyme activity. In some embodiments, the reaction product(s) are coupled with a mass tag, and the enzyme activity is determiner by analyzing the reaction product(s). The enzyme assays can be performed using mass spectrometry, for example nanostructure-initiator mass spectrometry (NIMS). Also described are methods, systems, and compositions for monitoring enzymatic degradation process of a substrate sample, for example a biomass.
Northen, Trent R (Walnut Creek, CA), Deng, Kai (Albany, CA), De Rond, Tristan P (Berkeley, CA), Peralta-Yahya, Pamela P (Emeryvill, CA), Cheng, Xiaoliang (Albany, CA), Keasling, Jay D (Berkeley, CA)
The Regents of the University of California (Oakland CA), Sandia Corporation (Livemore CA)
14/ 651,546
December 11, 2013
STATEMENT GOVERNMENT RIGHTS [0002] This invention was made with governmental support awarded by the National Institutes of Health under Grant No. 1RC1GM090980-01, and by the U.S. Department of Energy under Contract Nos. DE-AC02-05CH11231, DE-FC02-07ER64494, and DE-AC04-94AL85000. The government has certain rights in the invention. This work is also a collaboration between inventors at Lawrence Berkeley National Laboratory and Sandia National Laboratories for the Joint BioEnergy Institute.