The present invention provides novel methods for extraction of lipids from intact or lysed microorganisms in aqueous culture using a partially water soluble cosolvent with, or without a second organic solvent, and/or pressurized CO.sub.2 in the extraction methods. Such a process can also be implemented at a much larger industrial scale, where the economics of scale based capital expenditures costs distributed over much higher volume production as well as increased equipment efficiency would significantly improve production rates and lower costs.
STATEMENT OF GOVERNMENTAL INTEREST
 This invention was made with government support under grant no. DE-FG02-10ER85913 awarded by the Department of Energy. The government has certain rights in the invention.