A general methodology for highly sensitive and selective sensors that can achieve portable, low-cost and quantitative detection of a broad range of targets using only a personal glucose meter (PGM) is disclosed. The method and sensors take advantage of the ability of alkaline phosphatase (ALP) to convert glucose-1-phosphate to glucose, and the ability of PGMs to detect the generated glucose. The disclosed sensors can be part of a lateral flow device. Methods of using such sensors for detecting target agents, for example to diagnose disease, are also provided.
ACKNOWLEDGMENT OF GOVERNMENT SUPPORT
 This invention was made with government support under DE-FG02-08ER64568 awarded by the US Department of Energy, under ES 16865 awarded by the National Institutes of Health, and CTS-0120978 awarded by the National Science Foundation. The government has certain rights in the invention.