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United States Patent Application

View the Complete Application at the US Patent & Trademark Office
Provided herein are genetically engineered archaea. A genetically engineered archaea includes a heterologous polynucleotide that has a promoter operably linked to a coding region, where the coding region encodes a polypeptide having optimal activity below the optimum growth temperature (T.sub.opt) of the genetically engineered archaeon. Also provided herein are methods for using genetically engineered archaea and cell-free extracts of such genetically engineered archaea. In one embodiment, the methods include culturing a genetically engineered archaeon at a temperature that is at least C. below the T.sub.opt of the genetically engineered archaeon, such that the activity in the genetically engineered archaeon of a polypeptide encoded by the coding region is increased compared to the activity in the genetically engineered archaeon of the polypeptide during growth at a second temperature that is at or near the T.sub.opt of the genetically engineered archaeon.
Kelly, Robert M. (Raleigh, NC), Adams, Michael W.W. (Athens, GA), Basen, Mirko (Athens, GA), Lipscomb, Gina Pires (Athens, GA), Menon, Angeli (Athens, GA)
University of Georgia Research Foundation, Inc. (Athens GA), North Carolina State University (Raleigh NC)
14/ 355,362
November 2, 2012
GOVERNMENT FUNDING [0002] The present invention was made with government support under Grant No. DE-PS02-06ER64304 and Grant No. DE-AR0000081, each awarded by the Department of Energy, and under Grant No. BES-0617272, awarded by the National Science Foundation. The Government has certain rights in this invention.