Described herein are biosensors for early, pre-symptomatic detection of infectious agents and methods for their use. In particular, this disclosure describes biosensors that utilize toll-like receptor (TLR) binding domains to detect pathogen-associated molecular patterns (PAMPs). Also provided herein are methods of detecting and/or capturing a PAMP from a biological sample using the disclosed biosensors.
ACKNOWLEDGMENT OF GOVERNMENT SUPPORT
 This invention was made with government support under Contract No. DE-AC52-06NA25396 awarded by the U.S. Department of Energy. The government has certain rights in the invention.