A process is described for the creation of an inoculum for an organism capable of photosynthesis of a target compound within a closed bioreactor, involving steps of transfer of a sterile growth medium under pressure without exposure to atmosphere. The process is useful for the creation of inocula for the growth of cyanobacteria to produce target chemical products, such as ethanol, in closed photobioreactors.
STATEMENT REGARDING FEDERALLY SPONSORED RESEARCH
 This invention was made in part with United States government support under the Department of Energy grant number DE-EE0002867. The government has certain rights in this invention.